Conclusion - MaryamDost/GenomeAnalysis GitHub Wiki

The genome of Leptospirillum ferriphilum's type strain was assembled and annotated successfully. Specific genes related to its niche other expected genes were predicted and annotated on this genome. Genes responsible for e.g., nitrogen fixation, flagellar assembly and chemotaxis were identified. Synteny analyses showed well conserved genes within strains of L. ferriphilum, but less conserved in different species including same genus.

Transcriptome analyses was done using RNA-seq workflow. Differential expression analysis was also performed. Differential gene expression showed differenced in gene expression between chemostat cultures and bioleaching cultures. The overall results were reproduced successfully and were in coherent with the result presented in the paper.