Design of Linear Gates - LeonieZ/ACCEPT GitHub Wiki

In order to search for certain types of objects (like CTCs, Leukocytes, ...) you can design sets of linear gates that you can apply to the extracted measurements and visualize the results afterwards. You can call the window to design and apply the gates at multiple locations in the toolbox. The window that will open is shown below.

On the top you can set the name of the set and with the plus and minus signs you can adjust the number of lower and upper bounds that you use in your gate set.

For every bound you have to specify in which fluorescent channel you want to apply the gate, then choose in the drop-down list which feature you want to gate. Afterwards specify if it is a lower bound (click ">") or a higher bound ("<=") and then enter the threshold. Once you designed your gate you can either directly apply it by clicking "Done!" or save your gate first.

Saving of Gates: If you plan to use a gate more often it is most convenient to save it and reload it once you want to apply the gate to a new set of samples. To do so, click "Save". There is a default place to save the gates (the toolbox will always start looking for gates at this place) but you can also browse to wherever you want to save the gate and select "Save".

Loading of Gates: If you want to load a gate that you already saved (for example to make minor adjustments before applying it or to apply it as it is), click the load button and browse to your saved gates and select one. It is then visualized in the window for designing the gates.