Scaffolding Contigs with Hi‐C - Lavadav/EPP531_AGA GitHub Wiki
Scaffolding Slides
9. Juicer
mkdir Juicer
cd Juicer/
#Hap 1
mkdir hap1
cd hap1/
mkdir references
cd references/
ln -s </pwd/sassafrass_hap1.p_ctg.fasta> .
spack load /be6uuqp
bwa index sassafrass_hap1.p_ctg.fasta
cd ..
mkdir restriction_sites
cd restriction_sites/
python /pickett_shared/software/juicer-1.6/misc/generate_site_positions.py DpnII sassafrass_hap1.p_ctg ../references/sassafrass_hap1.p_ctg.fasta
awk 'BEGIN{OFS="\t"}{print $1, $NF}' sassafrass_hap1.p_ctg_DpnII.txt > sassafrass_hap1.p_ctg_DpnII.chrom.sizes
cd ..
mkdir fastq
cd fastq/
ln -s /path_to/salbidum01_1334141_S3HiC_R1.fastq.gz .
ln -s /path_to/salbidum01_1334141_S3HiC_R2.fastq.gz .
cd ..
bash /pickett_shared/software/juicer-1.6/CPU/juicer.sh \
-g sassafrass_hap1.p_ctg \
-D /pickett_shared/software/juicer-1.6/CPU/ \
-t 10 \
-d /path_to/Juicer/hap1/ \
-z ./references/sassafrass_hap1.p_ctg.fasta \
-p ./restriction_sites/sassafrass_hap1.p_ctg_DpnII.chrom.sizes \
-y ./restriction_sites/sassafrass_hap1.p_ctg_DpnII.txt