Mouse Transfer from Basement - KravitzLab/KreedLabWiki GitHub Wiki

Kreed lab procedure for bringing mice upstairs

  1. Log into Transnetyx.com, identify the mice you want and claim them using the "Labels" tag in the "cage" or "mouse" view (you can do this ahead of time, even right after genotyping). You can do this on Transnetyx by clicking on the top right three dots and click on assign owner.

  1. Check the 9th floor in-and-out or Kreed lab rack to ensure there is space for cages to move up (once you move them out of the breeding room they cannot go back!)

  2. Complete a transfer form utlizing KnowledgeLake on the WashuOne site. If you need a reminder, please check the github page here. (https://github.com/KravitzLab/KreedLabWiki/wiki/KnowledgeLake-E%E2%80%90Transfer-Forms).

  3. Go get your mice!

  4. After you move the mice, update the transnetyx website to reflect that you have moved mice out of the breeding room.

Required access

Everyone will need a login to transnetyx.com and a DCM tour to get access to our breeding room (360)

Breeding/weaning

Adrian/Sofia will manage breeding and weaning and will check weekly that transnetyx.com reflects the actual state of the breeding rack. Fridays will be the most up to date regarding the mice in the basement.

Potential failure points/other considerations:

• You can only move mice from the basement to the ninth floor. You cannot bring a cage from the ninth floor back to A360.

• People may forget to update Transnetyx when mice move out. Please send a Slack message to Adrian/Sofia if you didn't personally update transnetyx as they will update it on your behalf.

• People may claim mice on Transnetyx with the intention of using them but never get to it, blocking others from using them.

• People may forget to change their cages on the Kreed lab purple rack. Please include the your name, the experiment youre planning on using the mice for and the date of the initial start of the experiment.

• There is no substitute for communication – if you are claiming a bunch of mice ask around and see what other people's plans are.

• We can devote 5 minutes at lab meeting to review the weekly mouse moves, to help facilitate communication and make sure our breeding schemes are working.