I.P exendin. - KravitzLab/KreedLabWiki GitHub Wiki
Material needed :
- Exendin powder (Link)
- PBS
- Syringes
- Precision scale
- Pipette (50 uL)
- Ependorfs
- Saline
Use the 1 mg/mL stock (purple tube in -20 freezer) and make an intermediate dilution. You should dilute it to a ratio of 10 ųL stock : 990 ųL saline
Take the intermediate and dilute again, use 100 ųL intermediate : 900 ųL saline
Then dose the animals 0.1mL per gram (for example a 22 g animal would receive 0.22 mL drug)