/!\ /!\ WE HAVE 2 GRABDA VIRUSES, ONE IS A FIRST GEN RED SHIFTED AAV, ONE IS A 3RD GENE GREEN AAV, BE VERY CAREFUL WHICH ONE YOU ARE USING!!!

• Red shifted Grab DA : pAAV-hsyn-GRAB_rDA1m (https://www.addgene.org/140556/) ; Last ordered in august 2022.
• Green shifted Grab DA : pAAV-hSyn-GRAB-gDA3m (https://www.addgene.org/208698/); Last ordered in march 2025.

Both viruses work. Since the green shifted Grab DA is a 3rd generation, it is deemed more suitable for experiments than the red shifted virus.

Green shifted virus

Injection site

Virus : https://www.addgene.org/208698/

Serotype : AAV1

Volume : 360 nl

Dilution : No

Injection site : AP : 1.6 ; ML : 0.96 ; DV : -4.00

Photometry signal after six days

Time between surgeries and expression : around 2 weeks. Signal can start to be detected at day 6, if by day 15 no signal is detected it's unlikely that any recording will be possible with the mouse.

With amplification of the GrabDA through immunostaining

• Injection in the NAc : 360 nL ;
• Injecton in the DS : 360 nL ;
• IHF protocol : https://github.com/KravitzLab/KreedLabWiki/wiki/GFP-Staining

Red shifted virus

Virus : https://www.addgene.org/208698/

Serotype : AAV1

Volume : 360 nl

Dilution : No

Injection site : AP : 1.6 ; ML : 0.96 ; DV : -4.00

Photometry signal after 15 days