ChR2.md - KravitzLab/KreedLabWiki GitHub Wiki

Injection stereotaxic targeting: (VP) AP: 0.2, ML: 1.5, DV: -4.9

Virus construct: AAV5-eF1a-DIO-ChR2(H134R)-eYFP, titer: 1:4 dilution 6E10^12 vg/mL https://www.addgene.org/20298/

Injection volume: 300 nL, 50nL/60sec per cycle

Signal enhanced with 1:1000 chicken anti-GFP primary ab, 1:500 anti-chicken fluorescein secondary ab