Behavioral Recording Protocol (Bonsai)

Materials

• Mice
• Computer
• Wired circle
• 70% ethanol
• Light
• Camera

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Protocol

1. Open Bonsai on the computer.

2. Ensure Bonsai loads the correct protocol for video acquisition.

   • Confirm that the Suffix setting is set to time-stamp.

3. Prepare the video setup:

   • Clean the entire area using 70% ethanol.
   • Note: Mice can detect pheromones from the opposite gender, which may alter behavior.

4. When saving the file, ensure the format is .avi.

   • If not saved as .avi, the video will not record.
   • Use the naming format:
     YearMonthDate–mouseName
     Example: 20250120-MouseA

5. Start recording and adjust the camera focus on the bottom view.

   • The side camera is already auto focused.
   • Focus controls are located on the right-hand side.

6. Pause the video and delete the test recording.

   • The next video should now be properly focused.
   • Prepare a Post-it note with the mouse name and date.

7. Show the post-it note to the camera for labeling, then place the mouse into the chamber.

   • Record for 30 minutes. This timer is started once the door to the suite is closed.
   • Close the top portion of the chamber.

8. After 30 minutes, remove the mouse from the chamber and stop the recording.

   • Ensure the video files are saved to the correct behavioral computer directory.