Pisces Paper Analysis - Illumina/Pisces GitHub Wiki

This webpage details how to recreate the Pisces results from "Pisces: An Accurate and Versatile Variant Caller for Somatic and Germline Next-Generation Sequencing Data". The instructions are given using Docker, but they can easily be adapted to a local install. Your host machine will need a TB of space. ( If you want to skip Docker and run locally, you can use this analysis script as a guide and just change the directories as needed: run_analysis.sh )

1) Download the data

Download the required data (genome, bam files, and truth data) to the host machine, with the following directory structure. All .gz files should be unzipped.

[YourHostDataDir]/

2) Install and create the Docker image with both Pisces and Hap.py

(a) Install Docker, if you do not have it.

(b) Download Hap.py v0.3.10

(c) Replace the Hap.py docker file with the docker file located here: Docker image to rerun Pisces Paper analysis. This new Dockerfile fixes some issues with the standard Hap.py install, and installs Pisces on top of Hap.py. Copy run_analysis.sh into the same folder.

d) build the Docker image

docker build -t pisces .

3) Run Docker with the host data folder mounted at /data

docker run -it --mount type=bind,source=[YourHostDataDir]/,target=/data pisces /bin/bash

Running the docker image will set up the analysis software, and copy over the analysis script run_analysis.sh.

4) Run the analysis

Inside the docker container, run run_analysis.sh script. This script will generate the Pisces vcfs and the Hap.py / Som.py .csv files that give the precision and recall results, by sample. The numbers given in the paper are the average precision and recall, over all samples in a given dataset.