Yeast Standard Medium - IBEMicrobeHub/culturing GitHub Wiki
Materials:
- 2,5 mL of EtOH.
- 7,5 g of Agar 1,5 % (Ref. 214010, BD). Optional.
- Filters 0,22 µm.
- Yeast medium.
| Yeast Medium | Quantity |
|---|---|
| mQ water | 485 mL |
| Yeast extract (Ref. 212750, BD) | 2,5 g |
| Glycerol | 15 mL |
| K2HPO4 | 0,5 g |
Methhod:
- Prepare the Yeast medium (table).
- Autoclave it for 15 min at 121ºC.
- Add 2,5 mL of EtOH, and aseptically filter (0,22 µm) the medium.
- Store at 4ºC.
Optional: 7,5 g of agar 1.5 %.
Culture list
- Spizellomyces punctatus: 17ºC.