Salpingoeca minuta Medium - IBEMicrobeHub/culturing GitHub Wiki

Materials:

• 950 mL autoclaved & membrane filtered (pore size 0.2 µm) natural seawater.

Natural seawater can be replaced with high quality synthetic seawater made with Milli-Q water.

• 50 mL autoclaved distilled water.
• Organic enrichment (proteose peptone/yeast extract mixture) for Seawater medium.

Yeast extract mixture	Amounts
Proteose peptone	0,4 g
Yeast extract	0,08 g
Distilled water	100 mL

Method:

1. Add the natural seawater (950 mL) and the distilled water (50mL) together when they are cool.
2. Prepare the yeast extract mixture (table).
3. Dispense the organic aliquots into 2 mL Bijou bottles, autoclave and store them in a cool place.

For standard growth medium add 40 µL of peptone/yeast extract to 10 mL of seawater.