Seawater Rice Medium

Materials:

• Seawater or artificial seawater.
• Rice grains.
• Filters 3 µm.

Method:

1. Filter seawater (artificial seawater can be used instead) though a 3 µm membrane filter.
2. Autoclave for 15 min at 121ºC.
3. Let it cool and store at 4ºC.
4. Autoclave rice grains and store at room temperature.
5. Add 1 grain of rice for each 6 mL of culture.

Culture list:

• Diaphanoeca grandis: 4ºC.
• Stephanoeca diplocostata: 17ºC.

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Mineral Water Wheat Rice medium

Materials:

• Mineral water.
• Rice grains.

Method:

1. Autoclave mineral water for 15 min at 121ºC.
2. Let it cool and store at 4ºC.
3. Autoclave wheat rice grains and store at room temperature.
4. Add 1 grain of wheat rice for each 6 mL of culture.

Culture list:

• Nutomonas longa (formerly Planomonas carolina): 23ºC.
• Parabodo caudatus: 23ºC.