Marine Minimal Medium (adapted by JdC from MMM) - IBEMicrobeHub/culturing GitHub Wiki
Marine Minimal Medium (MMM) + 1 L of Eleven Salts Solution (ESS)
Ingredients:
| Elements | Amounts |
|---|---|
| 0,2% Glucosa (or an alternative carbon source) | 2 g |
| 9,52 mM NH4Cl | 0,51 g * |
| 1,32 mM K2HPO4 | 0,23 g |
| 40 mM MOPS | 8,3 g |
| Eleven Salts Solution | 1 L |
*0,952 mM para a medio limitado de N [0.05 g/1000 mL].
Method:
- Prepare the Eleven Salts Solution (ESS).
- Prepare the Marine Minimal Medium following the ingredients table.