Marine Broth 2216 ‐ Marine Agar 2216 - IBEMicrobeHub/culturing GitHub Wiki
Marine Broth 2216
Composition:
| Elements | Amounts |
|---|---|
| Peptone | 5 g |
| Yeast Extract | 1 g |
| Ferric Citrate | 0,1 g |
| Sodium Chloride | 19,45 g |
| Magnesium Chloride | 5,9 g |
| Magnesium Sulfate | 3,24 g |
| Calcium Chloride | 1,8 g |
| Potassium Chloride | 0,55 g |
| Sodium Bicarbonate | 0,16 g |
| Potassium Bromide | 0,08 g |
| Strontium Chloride | 0,034 g |
| Boric Acid | 0,022 g |
| Sodium Silicate | 0,004 g |
| Sodium Fluoride | 0,0024 g |
| Ammonium Nitrate | 0,0016 g |
| Disodium Phosphate | 0,008 g |
Materials and Methods:
- 37,4 g of Marine Broth powder (Ref. Marine Broth 2216, BD-Difco).
- 1L of distilled water.
- Filters 0,22 µm.
- Suspend 37,4 g of Marine Broth powder in 1 L of distilled water.
- Heat with frequent agitation and boil for 1 minute.
- Allow to cool and filter through 0,22µm membrane filter.
- Autoclave for 15 minutes at 121ºC.
- Store at 4ºC.
Marine Agar 2216
Composition:
| Elements | Amounts |
|---|---|
| Peptone | 5 g |
| Yeast Extract | 1 g |
| Ferric Citrate | 0,1 g |
| Sodium Chloride | 19,45 g |
| Magnesium Chloride | 8,8 g |
| Sodium Sulfate | 3,24 g |
| Calcium Chloride | 1,8 g |
| Potassium Chloride | 0,55 g |
| Sodium Bicarbonate | 0,16 g |
| Potassium Bromide | 0,08 g |
| Strontium Chloride | 0,034 g |
| Boric Acid | 0,022 g |
| Sodium Silicate | 0,004 g |
| Sodium Fluoride | 0,0024 g |
| Ammonium Nitrate | 0,0016 g |
| Disodium Phosphate | 0,008 g |
| Agar | 15 g |
Materials and Methods:
| Elements | Amounts |
|---|---|
| Yeast extract | 1 g |
| Triptone | 5 g |
| Agar | 15 g |
| Tap water | 1000 mL |
- Prepare the medium (1 L).
Half Concentrated Marine Agar
| Elements | Amounts |
|---|---|
| Marine Agar | 13,8 g |
| mQ Water | 500 mL |