MN Medium - IBEMicrobeHub/culturing GitHub Wiki

Ingredients:

  • 750 mL of sea water.
Solution I Amounts
Na2 EDTA 0,050 g
Citric acid 0,3 g
MgSO4·7H2O 3,8 g
H2O bd 100 mL
Solution II Amounts
NaH2PO4·H20 2 g
H2O bd 100 mL
Solution III Amounts
NaNO3 7,5 g
H2O bd 100 mL
Solution IV Amounts
Na2CO3 0,2 mg
H2O bd 100 mL
Solution V Amounts
CaCl2·H2O 1,8 g
H2O bd 100 mL
Solution VI Amounts
FeNH4(SO4)·12H2O (o citrate) 0,3 g
H2O bd 100 mL
H2SO4 Some drops
Micronutrients Amounts
H3BO3 0,286 g
MnCl2·4H20 0,181 g
ZnSO4·7H2O 0,022 g
CuSO4·5H2O 0,008 g
Na2MoO4·2H2O 0,039 g
CoCl2 0,005 g
NiCl2 0,001 g
H2O bd 100 mL
Cicloheximide solution Amounts
Cicloheximide 0,05 g
Methanol 75% 1 mL

Method:

  1. Add the following amounts of each solutions:
Solutions Amounts
Solution I 1 mL
Solution II 1 mL
Solution III 10 mL
Solution IV 10 mL
Solution V 1 mL
Solution VI 1 mL
Micronutrients 1 mL
Bidistilled water 225 mL
Sea water 750 mL
Cicloheximide solution 1mL
  1. Add 15 g of Agar for solid medium.