HL5‐C Medium - IBEMicrobeHub/culturing GitHub Wiki

*Materials:

• 26,5 g of HL5-C Medium (Ref. No. HLH1, Formedium).
• 1 L of distilled water.
• Filters 0,22 µm.

HL5-C Medium composition	Quantity
Yeast extract	7 g/L
Peptone	14 g/L
Potassium Dihydrogen Phosphate	0,5 g/L
Disodium Hydrogen Phosphate	0,5 g/L
Glucose	10 g/L
Supplemented with Vitamins & Micro-elements	0,1 g/L

Method:

1. Add 26,5 g of HL5-C Medium in 1 L of distilled water (previously autoclaved).
2. Filter.
3. Store at 4ºC.

Culture list

• Dictyostelium discoideum: 23ºC.