Eleven Salts Solution (adapted by JdC from NSS) - IBEMicrobeHub/culturing GitHub Wiki
Eleven Salts Solution [adapted by JdC from Nine Salts Solution (NSS)]
Ingredients:
- Sodium acetate 0,33% (3,3 g / 1000 mL)
- Fuctose.
- Octanoato.
| Elements | Amounts |
|---|---|
| NaCl | 17,6 g |
| NaSO4 | 1,47 g |
| NaHCO3 | 0,08 g |
| KCl | 0,025 g |
| KBr | 0,04 g |
| MgCl2·6H2O | 1,87 g |
| CaCl2·2H2O | 0,41 g |
| SrCl2·6H2O | 0,008 g |
| H3BO3 | 0,008 g |
| NaSiO3·9H2O | 0,005 g |
| FeCl3·6H2O | 0,00489 g |
Method:
- Prepare the solution following the table until the FeCl3·6H2O.
- Adjust the final pH at 8.
- Then add the sodium acetate, fructose and octanoato.