Brain Heart Infusion (BHI) diluted 1/10

Materials:

• 3,7 g of Brain Heart Infusion (BHI) (Ref. 237400, Becton Dickinson).
• 1 L of distilled water.
• Filter 0,22 µm.

Method:

1. Prepare the BHI concentrated version:

• Add 3,7 g of BHI in 1 L of distilled water.
• Autoclave for 15 min ar 121ºC.
• Filter the medium.
• Store at 4ºC.

2. Prepare the BHI diluted version (1/10):

• Autoclave 1 L of H2O.
• Under the biological hood, mix 100 mL of the BHI concentrated version with 900 mL of autoclaved H2O.

Culture list:

• Amoebidium parasiticum: 23ºC.