ATCC Medium: 802 (Cereal Grass Medium) - IBEMicrobeHub/culturing GitHub Wiki

Materials:

  • 2,5 g/L of Ward’s cereal grass powder (cat. no. 944 V 8602, Ward’s Natural Science).
  • 0,5 g Na2HPO4.
  • Distilled water.
  • Sterile deionised water.
  • Filter papers (Whatman #1).
  • Filters 0,22 µm.

Method:

  1. Add 2.5 mg/ mL Ward’s cereal grass powder to distilled water.

  2. Boil for 15 minutes.

  3. Allow to cool and filter through filter papers, followed by 0,22 µm membrane filter.

  4. Add 0,5 g of Na2HPO4.

  5. Autoclave for 15 min at 121ºC.

  6. Store at 4ºC.

Culture list

  • Parvularia atlantis: 23ºC.