ATCC Medium: 616 BG‐11 Blue‐Green Algae Medium - IBEMicrobeHub/culturing GitHub Wiki
ATCC Medium 616: BG-11 Medium for Blue-Green Algae
Ingredients:
| Elements | Amounts |
|---|---|
| NaNO3 | 1,5 g |
| K2HPO4 (or K2HPO4·2H2O) | 0,04 g (0,052 g) |
| MgSO4 7H2O | 0,075 g |
| CaCl2 2H2O (or CaCl2) | 0,036 g (0,027 g) |
| Citric acid | 0,006 g |
| Ferric ammonium citrate (or FeNH4(SO4)·12H2O) | 0,006 g (0,011 g) |
| EDTA | 0,001 g |
| NaCO3 | 0,02 g |
| Trace metal mix A5 (or Micronutrients solution) | 1 mL |
| Agar (if needed) | 10 g |
| Distilled water | 1 L |
| Trace metal mix A5 | Amounts |
|---|---|
| H3BO3 | 2,86 g |
| MnCl2·4H2O | 1,81 g |
| ZnSO4·7H2O | 0,222 g |
| NaMoO4·2H2O | 0,39 g |
| CuSO4·5H2O | 0,079 g |
| Co(NO3)2·6H2O | 0,0494 g |
| Distilled water | 1 L |
Method:
- Prepare the trace metal mix A5.
- Prepare the medium.
The pH should be 7,1 after sterilization.