AgendaJune2023 - I2PC/scipion-courses GitHub Wiki
Schedule and contents
*First Day:
[ * Extra 90 min: Welcome and presentation of the course, dynamics and mentors, introduction to scipion and aws. (if we are the first ones)]
-
11:30:
- Introduction to model building in cryo-EM (th-M)
- How to get a first estimation of the model (homology, alphafold, modelangelo) (th-M)
-
12:15:
- Presentation of the data used in the practical session
- Kahoot (R)
-
12:45 min: Map preprocessing (p-R)
-
13:30: lunch
-
14:30: getting a first estimate of the model (th-M)
-
16:00 coffee break
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16:30: Structure analysis: Structure comparison (M)
-
17:00: Rigid fitting of initial models (M)
-
18:00-30: End of the day
*Secons day:
- 9:30: Review of the previous day and kahoot. (M)
- 10:00: Flexible fitting I (R-coot)
- 11:00: Break
- 11:30: Flexible fitting (continuation) and validation (M-phenix)
- 13:30: lunch
- 14:30: Flexible fitting (continuation) and validation (cont) (M-phenix)
- 16:00: coffee break
- 16:30: Model building of the Beta sub-unit
- 18:00-30: end of the day
*third day:
- 9:30: Review of the previous day and kahoot. (M)
- 10:00: Presentation of the course questionnaire (R)
- 10:15: Building the model of the protein complex (M-join chains)
- 11:00: Coffee Break
- 11:30: Submission of structures (R-EMDB report) *
- 12:00: Structure analysis: Chain contacts (R)
- 13:00: INSTRUCT short message and farewell
- 13:30: lunch and end of the day