Restriction Enzyme Predictor - GenomicsCoreLeuven/GBSX GitHub Wiki

mandatory parameters:

• -d digest sequence

• `-l`     read length   
  

• `-f`     file of reference fasta file location(s)  
  

optional parameters:

• -e enzyme name to use (default: Enzyme)

• `-g`     genome name to use in bed file name (default: genome)  
  

• `-n`     minimum size fragments to include (default: 100)  
  

• `-m`     maximum size fragments to use (default: 1000)  
  

• `-E`     second enzyme name to use (default: Enzyme2)  
  

• `-D`     digest sequence for a second enzyme (default: not declared)  
  

• `-R`     digest sequence for a third enzyme (default: not declared)