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Manual binning with Anvi'o

Analyze real metagenomic data from unexplored cave microbiomes in the Democratic Republic of Congo. Learn to reconstruct microbial genomes from environmental samples and discover potentially novel organisms.

Course structure and time management

Total time: ~2.5 hours (plus assignment)

Section	Time	Description
01 Setting up	5 min	Connect to the supercomputer, request an interactive session and get the data
02 Installing Anvi'o for Metagenomics Analysis	20 min	Install Miniconda and Anvi'o and download databases
03 Explore input data	20 min	Explore different types of input files
04 Pre-binning	30 min	Gene detection and taxonomic estimations
05 Binning	30 min	Launch interactive interface and create 5 high-quality bins
Assignment	60 min	Multi-sample analysis: compare three cave locations