Dry sterilization using an oven (draft) - Cuddington-Lab/documents GitHub Wiki
Hazards:
Oven and hot material: Touching any internal parts of the oven or material placed in the oven during a dry autoclaving run may cause burns.
Steps:
- Cover all glasses with aluminum foil, leaving a small opening to allow gas exchange
- Wrap all tweezers with aluminum foil, ensuring a complete enclosure
- Ensure oven is off and cooled off
- Open the oven and place all the material inside
- Set oven to 180 °C for 3 hours, which allows for oven preheating, cooling down, and at least 1 hour of sterilization in high temperatures (https://www.cdc.gov/infectioncontrol/guidelines/disinfection/sterilization/other-methods.html)
How to use the oven:
- Keep the on/off button pressed for a few seconds for the oven to turn on
- Wait for the oven to test its functioning for about 4 times (it will display "Test" on the screen)
- Press "menu" to activate the menu bar, then ensure the red selection is on the thermometer
- Use the side arrows to select the right temperature
- Press "menu" to confirm
- Press "menu" again, and use the side arrows to select the timer (red selection)
- Select the amount of hours for the oven run
- Confirm using the "menu" button
- You are all done. The display will return to its default mode. In the menu bar, the "timer" icon will be illuminated and rotating
- The next day, ensure the oven is turned off and cooled down before opening its door (the door keeps automatically locked if the temperature is above 80 °C)
- Close tightly all of the aluminum foil coverings
- Remove the sterilized material from the oven
- Put material away in cabinets