Protocols_SOP_Template_V1 - BackmanLab/BackmanLab.github.io GitHub Wiki

Procedure: [Title]

SOP Prepared by:
Version: [start with 1.0, use the "decimals" to track small changes and the "units" to track major protocol changes]

  1. Scope

[one phrase describing what the protocol is used for].

The purpose of this SOP is to describe [xxxx]

  1. Description

[describe the process and what we want to accomplish by doing it].

This protocol aims to describe the procedure [xxxx].

  1. Personal Protective Equipment

[List the personal protective equipment to be used throughout the protocol and if applicable indicate additional equipment required for a particular step(s) identifying the step]

  1. Potential Hazards + Safety Precautions

[List all possible risks and safety measures to minimize them as well as what PPE to be used. If a particular reagent/set of reagents have specific hazards, identify the reagent, hazards and instructions for safe handling]

  1. Materials and Reagents

[List all the equipment, materials and reagents used to perform this protocol including vendor|brand, Cat#, and location information]

  1. Equipment

[List all equipment required to perform the Protocol in the below format]

= [Title] (Cont.) =

  • [Name] ([Brand], [Model], Cat#[xx] -- [location])

  1. Materials

[List all materials required to perform the Protocol in the below format]

  • [Name] ([Brand], [Model], Cat#[xx] -- [location])

  1. Reagents and Solutions

[List all reagents and solutions required to perform the Protocol in the below format. Indicate any specific hazards and handling instructions ]

  • [Concentration], [name] ([instructions for preparation] or

    [Brand], [concentration], Cat#[xx] -- [location])

[When applicable include***:***

NOTE #X: handling requirements (e.g. light sensitive, keep refrigerated, etc.). You can also re-list hazards (e.g. flammable, corrosive, carcinogenic, etc.), additional handling instructions or make a special call (e.g."make sure you read Sections 3 & 4 above before use")]

= [Title] (Cont.) =

  1. Procedure

[The Protocol can be numbered sequentially or subdivided in as many steps as you required. With each "step's title" in blue]

[Whenever relevant, document your protocol with visual aids.]

Title 1:

[include any relevant information the user should take in consideration prior to perform this step]

  1. [Number each step of the protocol with a number or bullet point]

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NOTE #: [Number the "Notes" sequentially. Include additional information relevant to the protocol | reagent | equipment use, here]

Title 2:

  5. ...

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  1. Disposals | Spills | Incidents

Waste (see waste segregations and collection SOP for complete information)

  • All waste containers should be closed once ¾ 's and placed into the appropriate location for pick up full.

  • All waste containers should be properly labelled with the following information:

    • PI's name

    • User | Responsible person

    • Content

    • Waste class (e.g. chemical, biohazard, radioactive, etc.)

  • = [Title] (Cont.) =

    • Waste type (e.g. sharps, plastic, glass, metal, etc.)

    • Waste "status" (e.g. liquid, solid, etc.)

[Protocol Specific]{.ul}

  1. Waste

    • [identify each of the protocol specific generated waste classes

      and instructions on how to discard it/them]

  2. Spills | Incidents:

[identify the type of possible spill|incident, the level of risk, and how to act in case it occurs].

  1. References

[List any support materials used to write the protocol].

= [Title] (Cont.) =

SUMMARY FLOWCHART OF PROCEDURES TO CONSIDER IN CASE OF A

xxxx EMERGENCY

[Only when applicable].

**
**

= [Title] (Cont.) =

// Protocol Summary//

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