The Small Molecules side of MSF performs targeted metabolomics and lipidomics analyses. Assays are available for absolute or relative quantitation for most panels. The established assays are performed on SCIEX QTRAP 6500 and 7500 mass spectrometers with Waters UHPLCs.

Additionally, Small Molecules performs pharmacokinetic-pharmacodynamic (PKPD) studies to determine the Adsorption, Distribution, Metabolism, and Elimination (ADME) of drugs of interest in order to improve their efficacy.

Below are links to key resources used by the Small Molecules team, including checklists, standard operating protocols (SOPs), and other reference material.

The lists below are key checklists used by the Small Molecules team for sample extraction and maintenance of instruments.

• Bligh-Dyer Extraction
• TRIzol-Bligh-Dyer Extraction
  • For high-biocontainment samples, such as BSL3 and BSL4 pathogens
• Methyl tert-butyl Ether (MTBE) Extraction

• Methanol Protein Precipitation

The lists below are the current assays in use by the Small Molecules side of the lab, separated by metabolomics and lipidomics.

• Targeted Metabolomics Panel
• Amino Acids and Analogues
• Nucleotides
• Sex Hormone Steroids
• Central Carbon Metabolites
• Organic Acids
• Bacterial Signaling Molecules (Alarmones)

• Targeted Lipidomics Panel (i.e. Lipidyzer 3.0)
• Fatty Acids
• Lipid Mediators
• Sterols and Cholesterol Esters
• Acylcarnitines
• Neutral Lipids
• Phosphatidic Acid Lipids