Research - coyotespike/LH GitHub Wiki

Hormones and Fluids

Human skin contains luteinizing hormone/chorionic gonadotropin receptors

"These data are the first demonstration of skin containing LH/hCG receptors and would suggest that LH and hCG may regulate skin functions."

• Prediction of ovulation

• Ovulation detection in the human.

• Localization of sex steroid receptors in human skin

• “Cervical Mucus Peroxidase Is a Reliable Indicator for Ovulation in Humans. - PubMed - NCBI.” Accessed July 20, 2016. http://www.ncbi.nlm.nih.gov/pubmed/6325254.

• “Natural Family Planning Indicators of Ovulation. - PubMed - NCBI.” Accessed July 21, 2016. http://www.ncbi.nlm.nih.gov/pubmed/3322540.

• “Luteinizing Hormone/human Chorionic Gonadotrophin Receptors in Various Epidermal Structures. - PubMed - NCBI.” Accessed July 21, 2016. http://www.ncbi.nlm.nih.gov/pubmed/10583046.

• “Sexual Hormones in Human Skin. - PubMed - NCBI.” Accessed July 21, 2016. http://www.ncbi.nlm.nih.gov/pubmed/17326004.

• "The Biological Actions of Estrogens on Skin. - PubMed - NCBI." Accessed July 21, 2016. http://www.ncbi.nlm.nih.gov/pubmed/12473056.

• Composition and Properties of Human Cervical Mucus

• “Cervical Mucus Peroxidase Is a Reliable Indicator for Ovulation in Humans. - PubMed - NCBI.” Accessed July 20, 2016. http://www.ncbi.nlm.nih.gov/pubmed/6325254. “jcinvest00340-0032.pdf.” Accessed July 26, 2016. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC293380/pdf/jcinvest00340-0032.pdf.

• Type E and Type G cervical fluids

• “{Salivary LH as an Ovulation Indicator: Comparison between Salivary LH, Serum LH and Ultrasonic Findings}. - PubMed - NCBI.” Accessed July 26, 2016. http://www.ncbi.nlm.nih.gov/pubmed/3319762.

Testing Methods and Sensors

• “Measurement of LH in Saliva: A New Approach to Ovulation Detection. | POPLINE.org.” Accessed July 26, 2016. http://www.popline.org/node/419010.

• Enzyme Immunoassay for the Quantitative Determination of LH

• “New Nanoscale Biosensor for Detection of Luteinizing Hormone in Small Ruminants to Determine Optimum Breeding Time - LINCOLN UNIVERSITY.” Accessed July 26, 2016. http://www.reeis.usda.gov/web/crisprojectpages/0223922-new-nanoscale-biosensor-for-detection-of-luteinizing-hormone-in-small-ruminants-to-determine-optimum-breeding-time.html.

• “A Nanosensor Platform for Direct Detection of a Cancer Biomarker in Blood.” Accessed July 27, 2016. http://www.nanowerk.com/spotlight/spotid=20257.php.

• “Transport across Artificial Membranes–an Analytical Perspective - Springer.” Accessed July 27, 2016. http://link.springer.com/article/10.1007%2Fs00216-006-0305-9#/page-1.

• Nanotech LH sensor, but looks just like an inconvenient chemical test

• “Saliva-Based Biosensors: Noninvasive Monitoring Tool for Clinical Diagnostics.” Accessed July 27, 2016. http://www.hindawi.com/journals/bmri/2014/962903/.

• “Cervical Mucus: Present State of Knowledge. | POPLINE.org.” Accessed July 26, 2016. http://www.popline.org/node/502220.

• Content of cervical mucus

• [Optics detect antibodies and human hormones] (http://digital.csic.es/bitstream/10261/28001/1/GetPDFServlet.pdf)

• [2003, Microfluidic Chip for Continuous Monitoring of Hormone Secretion from Live Cells Using an Electrophoresis-Based Immunoassay] (http://pubs.acs.org/doi/abs/10.1021/ac0346813)

Reasons we think the LH surge may affect human skin

The skin has LH receptors

“Luteinizing Hormone/human Chorionic Gonadotrophin Receptors in Various Epidermal Structures. - PubMed - NCBI.” Accessed July 21, 2016. http://www.ncbi.nlm.nih.gov/pubmed/10583046.

The skin behaves differently during the luteal phase

Rhiannon: adult acne outbreaks usually occur in the luteal phase, following the LH surge.

Perspiratory system is part of your excretory system, and we know LH is detectable in urine.

Background Info on LH

Produced by gonadotropic cells in the anterior lobe of the pituitary gland. The pituitary gland is located on the underside of the brain. An LH surge occcurs about day 14 of a 28-day cycle. In men, LH stimulates testosterone (and through testosterone, sperm production).

Currently, for a woman, more than one blood sample may be needed. Also urine tests.

Testosterone is made in the interstitial or Leydig cells, between the seminiferous tubules. The interstitial cells are targets for interstitial cell stimulating hormone, ICSH, which is LH.

An ovarian follicle is the basic unit of female reproductive biology; each contains a single oocyte, an immature egg cell or ovum.

When the hypothalumus sends gonadotropin-releasing hormone (GnRH) to the anterior lobe of the pituitary, follicle-stimulating hormone (FSH) is synthesized and released. Assisted by LH, FSH causes the follicle to release estrogens.

LH is synthesized at the same location and also as a result of GnRH, and is also a heterodymeric glycoprotein (two different proteins, with a carbohydrate attached, making the glycoprotein very hydrophilic). It has the same 92-amino acid alpha subunit as in FSH, and a beta chain of 121 amino acids which cause LH's unique properties.

The polyeptide LHβ is encoded by a single gene in all mammals; in primates this gene is located within a cluster that arose through gene duplication.

The LH surge triggers the completion of meiosis I of the egg, and its release (ovulation) in mid-cycle.

E3G is produced when estrogen breaks down, accumulating in urine around the time of ovulation and causing the cervical mucus to become thin and slippery. This may increase the chance of pregnancy.