Versatile Volume Data (VVD) Viewer is an interactive rendering tool for visualizing and analyzing confocal microscopy data. This wiki explains the features of VVDViewer and provides tips for getting the most out of the software. Using the menu to the right you can learn to:

• Navigate all the windows, tools, menus, and settings available in VVDViewer.
• View and manipulate volume data to optimize visualization.
• Make figures and movies
• Perform analyses such as segmentation and particle detection

VVDViewer combines rendering of multi-channel volume data and polygon mesh (surface) data and allows the properties of each channel and dataset to be adjusted quickly and independently. The data can then be combined into a single 3D image showing the relationship between individually labeled structures (e.g., neurons) without background noise. The tool is designed for neurobiologists to better visualize confocal samples stained with fluorescent markers.

With VVDViewer, you get excellent rendering quality with three different render modes (composite, depth, layer), interactive 3D segmentation, and 3D visualization of huge chunked datasets (>100GB).

• Click to download the latest version of VVD for your operating system:

Older versions of VVDViewer might not be compatible with the latest Mac OS, Sonoma. If you encounter any issues on Sonoma, please ensure that VVDViewer is updated to the most recent version.

If you get Vulkan-related errors, please try to update your graphics device driver.

Click here to watch the quick start video:

4D zebrafish eye development 1.3GB

If you use this data, please cite the following paper:
"A complex choreography of cell movements shapes the vertebrate eye."
Kristen M. Kwan, Hideo Otsuna, Hinako Kidokoro, Keith R. Carney, Yukio Saijoh, Chi-Bin Chien. Development (2012) 139 (2): 359–372.

Drosophila brain & neurons 59.5MB

Six examples of Drosophila brain data published in this manuscript:
"A searchable image resource of Drosophila GAL4-driver expression patterns with single neuron resolution." Meissner GW, Nern A, Dorman Z, Depasquale GM, Forster K, Gibney T, Hausenfluck JH, He Y, Iyer NA, Jeter J, Johnson L, Johnston RM, Lee K, Melton B, Yarbrough B, Zugates CT, Clements J, Goina C, Otsuna H, Rokicki K, Svirskas RR, Aso Y, Card GM, Dickson BJ, Ehrhardt E, Goldammer J, Ito M, Kainmueller D, Korff W, Mais L, minegishi r, Namiki S, Rubin GM, Sterne GR, Wolff T, Malkesman O. eLife. 2023 Feb 23;12. doi: 10.7554/eLife.80660

• Readme file providing information on downloading and citing VVDViewer and links to source code.
• We are happy to support you. Please write comments and discussion here in image.sc forum.

We are proud to announce that VVDViewer is now a Community Partner with image.sc, a forum for scientific image software. We are looking forward to contributing to and benefiting from this vibrant community.